MicroRNA-101 inhibits the proliferation and invasion of bladder cancer cells via targeting c-FOS.

MicroRNAs (miRs) have important roles in the parthenogenesis of malignancies. While it has been suggested that deregulation of miR-101 is involved in bladder cancer, the underlying mechanisms have remained largely elusive. The present study aimed to investigate the roles of miR-101 in the regulation of bladder cancer cell proliferation and invasion. Reverse-transcription quantitative polymerase chain reaction analysis revealed that the expression of miR-101 was significantly reduced in the HT-1376, BIU87, T24 and 5637 several human bladder cancer cell lines compared to that in the SV-HUC-1 normal bladder epithelial cell line. Furthermore, a Targetscan search and a luciferase assay were used to identify c-FOS as a novel target of miR-101, and western blot analysis indicated that the protein expression of c-FOS was shown to be negatively regulated by miR-101 in bladder cancer T24 cells; however, c-FOS mRNA expression was not affected. In addition, plasmid-mediated overexpression of miR-101 and small hairpin RNA-mediated inhibition of c-FOS significantly inhibited the proliferation and invasive capacity of T24 cells, as indicated by an MTT and a Transwell assay, respectively. However, plasmid-mediated overexpression of c-FOS reversed the inhibitory effects of miR-101 overexpression on T24-cell proliferation and invasion. In conclusion, the present study demonstrated that miR-101 inhibits the proliferation and invasion of bladder cancer cells, at least partly via targeting c-FOS, suggesting that miR-101/c-FOS signaling may represent a potential therapeutic target for bladder cancer. [ABSTRACT FROM AUTHOR]