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Brief communication TNF-α promoter polymorphisms and extended HLA and TNF-α haplotypes in Koreans based on 100 families.
- Source :
-
Tissue Antigens . Jan2004, Vol. 63 Issue 1, p75-79. 5p. - Publication Year :
- 2004
-
Abstract
- We investigated five single-nucleotide polymorphism (SNP) sites of tumor necrosis factor-α (TNF-α) promoter, the distribution of five-locus TNF-α haplotypes, and extended human leukocyte antigen (HLA)/TNF-α haplotypes in 200 parents from 100 unrelated Korean families. TNF-α SNPs were typed using polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP) and PCR restriction fragment length polymorphism (PCR-RFLP) methods. The frequencies of −1031C, −863A, −857T, −308A, and −238A alleles were 0.185, 0.145, 0.140, 0.060, and 0.040, respectively. The allele frequencies in Koreans were similar to those of Japanese. A total of five TNF-α haplotypes (−1031/−863/−857/−308/−238) were detected in 196 parents: TCCGG (frequency 0.617), CACGG (0.143), TCTGG (0.143), TCCAG (0.061), and CCCGA (0.036). Five most common extended HLA/TNF-α haplotypes (frequencies >0.020) accounted for 16% (63/392) of the total haplotypes. A total of 223 HLA haplotypes and 239 HLA/TNF-α haplotypes were detected, indicating that most HLA haplotypes are exclusively associated with particular TNF-α haplotypes. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00012815
- Volume :
- 63
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Tissue Antigens
- Publication Type :
- Academic Journal
- Accession number :
- 11784437
- Full Text :
- https://doi.org/10.1111/j.1399-0039.2004.00148.x