Efficient purification and complete NMR characterization of galactinol, sucrose, raffinose, and stachyose isolated from Pinus halepensis (Aleppo pine) seeds using acetylation procedure.

The use of precipitation followed by acetylation procedures and preparative TLC purification allowed a facile isolation of four carbohydrates from the methanol extract ofPinus halepensisseeds. The isolated oligosaccharides exhibited high degree of purity. They were identified as α-D-galactosyl-(1→1)-myo-inositol nonaacetate (1), α-D-glucosyl-(1→2)-β-D-fructosyl octaacetate (2), α-D-galactosyl-(1→6)-α-D-glucosyl-(1→2)-β-D-frutosyl undecaacetate (3), and α-D-galactosyl-(1→6)-α-D-galactosyl-(1→6)-α-D-glucosyl-(1→2)-β-D-frutosyl tetradecaacetate (4) and were isolated for the first time from this plant. The1H and13C NMR assignments for compounds2,3, and4were detailed herein for the first time. [ABSTRACT FROM PUBLISHER]