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Wnt3a nanodisks promote ex vivo expansion of hematopoietic stem and progenitor cells.
- Source :
-
Journal of Nanobiotechnology . 8/23/2016, Vol. 14, p1-10. 10p. - Publication Year :
- 2016
-
Abstract
- Background: Wnt proteins modulate development, stem cell fate and cancer through interactions with cell surface receptors. Wnts are cysteine-rich, glycosylated, lipid modified, two domain proteins that are prone to aggregation. The culprit responsible for this behavior is a covalently bound palmitoleoyl moiety in the N-terminal domain. Results: By combining murine Wnt3a with phospholipid and apolipoprotein A-I, ternary complexes termed nanodisks (ND) were generated. ND-associated Wnt3a is soluble in the absence of detergent micelles and gel filtration chromatography revealed that Wnt3a co-elutes with ND. In signaling assays, Wnt3a ND induced β-catenin stabilization in mouse fibroblasts as well as hematopoietic stem and progenitor cells (HSPC). Prolonged exposure of HSPC to Wnt3a ND stimulated proliferation and expansion of Lin- Sca-1+ c-Kit+ cells. Surprisingly, ND lacking Wnt3a contributed to Lin- Sca-1+ c-Kit+ cell expansion, an effect that was not mediated through β-catenin. Conclusions: The data indicate Wnt3a ND constitute a water-soluble transport vehicle capable of promoting ex vivo expansion of HSPC. [ABSTRACT FROM AUTHOR]
- Subjects :
- *WNT proteins
*STEM cells
*CELL receptors
*GEL permeation chromatography
*CATENINS
Subjects
Details
- Language :
- English
- ISSN :
- 14773155
- Volume :
- 14
- Database :
- Academic Search Index
- Journal :
- Journal of Nanobiotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 117641256
- Full Text :
- https://doi.org/10.1186/s12951-016-0218-5