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Mono- Versus Polyubiquitination: Differential Control ofp53 Fate by Mdm2.
- Source :
-
Science . 12/12/2003, Vol. 302 Issue 5652, p1972-1975. 4p. 2 Color Photographs, 1 Diagram. - Publication Year :
- 2003
-
Abstract
- Although Mdm2-mediated ubiquitination is essential for both degradation and nuclear export of p53, the molecular basis for the differential effects of Mdm2 remains unknown. Here we show that low levels of Mdm2 activity induce monoubiquitination and nuclear export of p53, whereas high levels promote p53's polyubiquitination and nuclear degradation. A p53-ubiquitin fusion protein that mimics monoubiquitinated p53 was found to accumulate in the cytoplasm in an MdmZ-independent manner, indicating that monoubiquitination is critical for p53 trafficking. These results clarify the nature of ubiquitination-mediated p53 regulation and suggest that distinct mechanisms regulate p53 function in accordance with the levels of Mdm2 activity. [ABSTRACT FROM AUTHOR]
- Subjects :
- *UBIQUITIN
*NUCLEAR nonproliferation
*CYTOPLASM
*CELLS
*TUMOR suppressor proteins
Subjects
Details
- Language :
- English
- ISSN :
- 00368075
- Volume :
- 302
- Issue :
- 5652
- Database :
- Academic Search Index
- Journal :
- Science
- Publication Type :
- Academic Journal
- Accession number :
- 11762272