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Strand-invading linear probe combined with unmodified PNA.
- Source :
-
Bioorganic & Medicinal Chemistry . Sep2016, Vol. 24 Issue 18, p4129-4137. 9p. - Publication Year :
- 2016
-
Abstract
- Efficient strand invasion by a linear probe to fluorescently label double-stranded DNA has been implemented by employing a probe and unmodified PNA. As a fluorophore, we utilized ethynylperylene. Multiple ethynylperylene residues were incorporated into the DNA probe via a d -threoninol scaffold. The ethynylperylene did not significantly disrupt hybridization with complementary DNA. The linear probe self-quenched in the absence of target DNA and did not hybridize with PNA. A gel-shift assay revealed that linear probe and PNA combination invaded the central region of double-stranded DNA upon heat-shock treatment to form a double duplex. To further suppress the background emission and increase the stability of the probe/DNA duplex, a probe containing anthraquinones as well as ethynylperylene was synthesized. This probe and PNA invader pair detected an internal sequence in a double-stranded DNA with high sensitivity when heat shock treatment was used. The probe and PNA pair was able to invade at the terminus of a long double-stranded DNA at 40 °C at 100 mM NaCl concentration. [ABSTRACT FROM AUTHOR]
- Subjects :
- *DNA analysis
*DNA probes
*ANTHRAQUINONES
*HEAT shock factors
*QUENCHING (Chemistry)
Subjects
Details
- Language :
- English
- ISSN :
- 09680896
- Volume :
- 24
- Issue :
- 18
- Database :
- Academic Search Index
- Journal :
- Bioorganic & Medicinal Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 117518213
- Full Text :
- https://doi.org/10.1016/j.bmc.2016.06.055