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Community composition and activity of anaerobic ammonium oxidation bacteria in the rhizosphere of salt-marsh grass Spartina alterniflora.
- Source :
-
Applied Microbiology & Biotechnology . Sep2016, Vol. 100 Issue 18, p8203-8212. 10p. - Publication Year :
- 2016
-
Abstract
- Anaerobic ammonium oxidation (anammox) as an important nitrogen removal pathway has been investigated in intertidal marshes. However, the rhizosphere-driven anammox process in these ecosystems is largely overlooked so far. In this study, the community dynamics and activities of anammox bacteria in the rhizosphere and non-rhizosphere sediments of salt-marsh grass Spartina alterniflora (a widely distributed plant in estuaries and intertidal ecosystems) were investigated using clone library analysis, quantitative PCR assay, and isotope-tracing technique. Phylogenetic analysis showed that anammox bacterial diversity was higher in the non-rhizosphere sediments ( Scalindua and Kuenenia) compared with the rhizosphere zone (only Scalindua genus). Higher abundance of anammox bacteria was detected in the rhizosphere (6.46 × 10-1.56 × 10 copies g), which was about 1.5-fold higher in comparison with that in the non-rhizosphere zone (4.22 × 10-1.12 × 10 copies g). Nitrogen isotope-tracing experiments indicated that the anammox process in the rhizosphere contributed to 12-14 % N generation with rates of 0.43-1.58 nmol N g h, while anammox activity in the non-rhizosphere zone contributed to only 4-7 % N production with significantly lower activities (0.28-0.83 nmol N g h). Overall, we propose that the rhizosphere microenvironment in intertidal marshes might provide a favorable niche for anammox bacteria and thus plays an important role in nitrogen cycling. [ABSTRACT FROM AUTHOR]
- Subjects :
- *AMMONIUM
*NITROGEN
*RHIZOSPHERE
*SPARTINA alterniflora
*POLYMERASE chain reaction
Subjects
Details
- Language :
- English
- ISSN :
- 01757598
- Volume :
- 100
- Issue :
- 18
- Database :
- Academic Search Index
- Journal :
- Applied Microbiology & Biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 117510210
- Full Text :
- https://doi.org/10.1007/s00253-016-7625-2