二氧化钛纳米材料对氧化应激状态大鼠肾脏的毒性.

Objective To investigate the adverse effects of nano-titanium dioxide (TiO2) on kidney tissues in healthy rats and rats with oxidative stress (OS). Methods Thirty-six healthy male SD rats were randomly divided into control group, alloxan-treated group, nano-TiO2 treated (NM) group, and alloxan and nano-TiO2 dual treatment (OS-NM) group. Nano-TiO2 of three concentrations (0.5, 5 and 50mg/kg body weight) was injected intraperitoneally. The level of OS biochemical factors and blood urea nitrogen (BUN) and pathological changes of kidney were determined. Results Compared with those in NM and OS groups, the levels of O2-· and superoxide dismutase (SOD) in OS-NM group were significantly increased after exposure to nano-TiO2 of 5 mg/kg body weight (P<0.05). Nano-TiO2 of 50mg/kg body weight led to significant changes of O2-·, SOD, and glutathione (GSH) levels in OS-NM group in comparison with OS and NM groups (P<0.01). The levels of O2-· and GSH between OS group and NM group changed significantly (P<0.05). Compared with healthy rats, OS rats showed significant increased BUN level (P<0.01), cell number and edema of renal tubular epithelial cells after nano-TiO2 exposure. A synergic effect between OS condition and nano-TiO2 level was shown on the increased level of O2-·. Conclusion Nano-TiO2 induced more adverse effects on the kidney in OS rats, suggesting a synergistic effect between nano-TiO2 and OS. This result provides experimental evidence for patients’ safe use of nano-TiO2. [ABSTRACT FROM AUTHOR]