重组异种骨对Balb/c小鼠的免疫学影响.

Objective To make a dynamic evaluation of the immunotoxicology caused by heterogeneous bone implantation and reveal its immunological mechanisms. Methods Heterogeneous bone was provided by Guangdong Grandhope Biotech Co., Ltd. Immunology of Balb/c mice by blood biochemical analysis, ELISA and flow cytometry after the test of lymphocyte transformation in vitro. We dynamically monitored the immunological changes Balb/c mice after 1 week, 2 weeks and 4 weeks of muscle implantation to evaluate the immunological effect of the heterogeneous bone on the body. Results Our experiments showed that the heterogeneous bone did not cause immune stimulation of lymphocytes in vitro, and there were no differences between implantation of heterogeneous bone and the sham operation in the levels of C3, IgG, IgM, inflammatory factors (TNF-α and IL-6) in serum and ALP in implantation site. Moreover, heterogeneous bone implantation did not affect the proportion or activation of peripheral blood mononuclear cells (PBMCs) and lymphocytes in the lymph node. Conclusion The heterogeneous bone cannot mediate immunotoxicology in vivo, which may provide experimental data and theoretical basis for clinical application of heterogeneous bone. [ABSTRACT FROM AUTHOR]