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Direct ethanol fermentation of the algal storage polysaccharide laminarin with an optimized combination of engineered yeasts.
- Source :
-
Journal of Biotechnology . Aug2016, Vol. 231, p129-135. 7p. - Publication Year :
- 2016
-
Abstract
- Laminarin is the algal storage glucan and represents up to 35% of the dry weight of brown macroalgae. In this study, a novel laminarinase, Gly5M, was first found using focused proteome analysis of a laminarin-assimilating marine bacterium, Saccharophagus degradans , and the encoding gene was isolated. A Gly5M-displaying yeast strain was prepared with the cell surface display system using Saccharomyces cerevisiae . It showed a laminarin-degrading activity on the cell surface and caused the dominant accumulation of gentiobiose. The obtained gentiobiose was converted into glucose and could be assimilated by an Aspergillus aculeatus β-glucosidase (BG)-displaying yeast strain. When Gly5M- and BG-displaying yeasts were anaerobically cultivated together in fermentation medium containing 20 g/L laminarin as a sole carbon source, the coculture system with the combination of optimized ratios of the 2 yeast strains directly produced 5.2 g/L ethanol. This coculture system of the 2 engineered yeast strains would be a platform for the use of laminarin and contribute to the complete utilization of brown macroalgae. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 01681656
- Volume :
- 231
- Database :
- Academic Search Index
- Journal :
- Journal of Biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 117011024
- Full Text :
- https://doi.org/10.1016/j.jbiotec.2016.06.002