万古霉素/磷酸钙骨水泥复合材料的细胞毒性研究.

Objective: To investigate the cytotoxicity of vancomycin(Van)/calcium phosphate cement(CPC) composite materials on rat bone marrow mesenchymal stem cells(BMSCs). Methods: Primary BMSCs were isolated, cultured and identified. The cells were incubated with different concentrations(containing 0%, 1%, 5%, 10% Van) of Van/CPC composite materials. The cell viability of BMSCs was measured by CCK-8 method, and the cell apoptosis was detected by TUNEL method and the cell morphology was observed-by scanning electron microscope(SEM). Results: 5% and 10% Van/CPC composite materials could significantly inhibit the proliferation of BMSCs(P<0.05), and increase apoptosis(P<0.05) and lead to higher cytotoxicity.1% Van/CPC exerted no obvious effect on the BMSCs compared to the blank control. Conclusion: Low concentration of Van/CPC had no cytotoxicity on rat and BMSCs performed good biocompatibility. [ABSTRACT FROM AUTHOR]