动脉钙化大鼠动脉组织 TGF-β、MMPs 表达变化及意义.

Objective To explore the potential role of transforming growth factor-β (TGF-β) and matrix metalloproteinases (MMPs) in its occurrence and development of arterial calcification. Methods Sixty male SD rats were randomly divided into the control group and experimental group, with 30 rats in each group. Rats in the experimental group were injected vitamin D3 with a dose of 500 000 U/(kg· d) subcutaneously for 3 consecutive days , which was in order to induce the arterial calcification in rat models. The same dose of 5% glucose without vitamin D3 was injected into the rats of two groups subcutaneously. Using Von Kossa staining to observe the degree of tissue calcification of the rats, and using real-time fluorescent quantitative PCR to determinate the expression of mRNA of TGF-β and MMPs in the two groups. Results The degree of calcification and the mRNA expression of TGF-β and MMPs in the experimental group were significantly higher than those of the control group (all P<0.05). Conclusion The TGF-β and MMPs may contribute to the formation of arterial calcification. [ABSTRACT FROM AUTHOR]