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Genetic improvement of the nematicidal fungus Lecanicillium attenuatum against Heterodera glycines by expression of the Beauveria bassiana Cdep1 protease gene.
- Source :
-
Journal of Invertebrate Pathology . Jul2016, Vol. 138, p86-88. 3p. - Publication Year :
- 2016
-
Abstract
- Lecanicillium attenuatum is an important nematophagous fungus with potential as a biopesticide against plant-parasitic nematodes. The Pr1A-like cuticle-degrading protease ( Cdep1 ) gene originating from the entomopathogenic fungus Beauveria bassiana was transformed into the nematophagous fungus L. attenuatum using a polyethylene-glycol mediated protoplast-based transformation system. Protease activity was increased 0.64- to 1.63-fold 2–10 d after growth in the transformed L. attenuatum . Inhibition of egg-hatching and J2 motility of soybean cyst nematodes ( Heterodera glycines ) by cell-free fungal culture filtrates were enhanced by 17–76% 2–14 d and 43–152% 1–13 d after incubation, respectively. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00222011
- Volume :
- 138
- Database :
- Academic Search Index
- Journal :
- Journal of Invertebrate Pathology
- Publication Type :
- Academic Journal
- Accession number :
- 116765131
- Full Text :
- https://doi.org/10.1016/j.jip.2016.06.008