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A polysaccharide from the stems of Rubus amabilis Focke and its immunological enhancement activity.
- Source :
-
Journal of Asian Natural Products Research . Sep2016, Vol. 18 Issue 9, p897-907. 11p. - Publication Year :
- 2016
-
Abstract
- A water-soluble polysaccharide (named RAP) was newly isolated from the stems ofRubus amabilis. Structural confirmation of the polysaccharide was provided by hydrolysis, periodate oxidation, Smith degradation, and methylation analysis, combined with nuclear magnetic resonance (NMR), capillary electrophoresis (CE), infrared spectroscopy (IR), and gas chromatography-mass spectra (GC-MS).In vitroimmunological enhancement activity was characterized using the proliferative activity of spleen lymphocytes and phagocytic activity of peritoneal macrophages in mice. The polysaccharide was mainly composed of xylose, arabinose, glucose, rhamnose, galactose, mannose, glucuronic acid, and galactocuronic acid in the molar ratio of 1.0:6.9:0.8:1.1:6.9:0.3:0.5:3.3, with the average molecular weight of 26.2 kDa. The linkage types of netural monosaccharides were as follows: the arabinose was →2) Ara (1→ and galactose were Gal (1→, →3) Gal (1→, →3,6) Gal (1→, →2,3,6) Gal (1→ and →2,3,6) Galf(1→. Xyl (1→, →6) Glc (1→, →2) Glc (1→, →3) Rha (1→, Rha (1→ and Man (1→ were also found in the structure. RAP-B-2 could improve the proliferative activity of spleen T cells and B cells and boost phagocytic activity of peritoneal macrophages at the concentration of 50 μg/ml (p < 0.05,p < 0.01). [ABSTRACT FROM PUBLISHER]
- Subjects :
- *ALTERNATIVE medicine
*ANIMAL experimentation
*BIOLOGICAL models
*CELL physiology
*PHYSICAL & theoretical chemistry
*DOSE-effect relationship in pharmacology
*ELECTROPHORESIS
*GAS chromatography
*HIGH performance liquid chromatography
*IMMUNE system
*LYMPHOCYTES
*MACROPHAGES
*MASS spectrometry
*MEDICINAL plants
*METHYLATION
*MICE
*NUCLEAR magnetic resonance spectroscopy
*POLYSACCHARIDES
*PROBABILITY theory
*REGRESSION analysis
*RESEARCH funding
*SPECTROPHOTOMETRY
*SPLEEN
*PLANT stems
*PLANT extracts
*DESCRIPTIVE statistics
*IN vitro studies
Subjects
Details
- Language :
- English
- ISSN :
- 10286020
- Volume :
- 18
- Issue :
- 9
- Database :
- Academic Search Index
- Journal :
- Journal of Asian Natural Products Research
- Publication Type :
- Academic Journal
- Accession number :
- 116647091
- Full Text :
- https://doi.org/10.1080/10286020.2016.1173675