"99Ｔc-ＭＤＰ" 治疗股骨头坏死的实验研究.

Objective To investigate the value of 99Tc-MDP therapy in aseptic necrosis femur head (ANFH). Methods The animal experiment was divided into 2 stages: animal model establishment and clinical observation. In the first stage, Group A (normal group) and Group B(ANFH group) were set up. The animal ANFH model was established by intravenous injection of dexamethasone sodium phosphate for 8 weeks. Bone pathology, histomorphology, bone mineral density, tetracycline double labeling experiment, radionuclide bone scintigraphy ROI ratio value, X-ray, and CT were examined. The second stage of the experiment started until the ANFH model had been proved. During the second stage, these groups were set up for the further 16 week-therapy based on the ANFH animal model: Group C (normal control), Group D(ANFH control), Group E(99Tc-MDP therapy), and Group F(alendronate therapy). After the treatment, the efficacy in Group E and Group F was valued. A SPPSS13. 0 softw are was used for statistical analysis. Results After 2 stages of ANFH model establishment and clinical observation, the results, including bone pathology, histomorphology, bone mineral density, tetracycline double labeling experiment, and ROI ratio of radionuclide bone scintigraphy, show ed apparent difference between Group A and B(p < 0. 01), indicating the success of animal modeling. After the treatment, the bone pathology in Group E show ed that trabecular bone in the femoral head lined regularly with a little scatter. But the repair was apparent comparing with that in Group D. In Group F trabecular bone in the femoral head show ed scattered and irregular lining and little broken. Comparing with that in Group D, the situation improved in Group F. The results of bone mineral density, tetracycline double labeling experiment, and ROI ratio of radionuclide bone scintigraphy also supported the improvement(p < 0. 05). Conclusion On the bases of ANFH animal model, 99Tc-MDP and Alendronate have got different therapy value and99Tc-MDP show ed an apparent remoulding result. 99Tc-MDP can reach the ANFH target tissue. Besides controlling bone absorption, it also can maintain the activity of superoxide dismutase(SOD), protect the bone tissue absorption from free radius and prevent from its further damaging. From the tetracycline double labeling experiment we can conclude that the increased deposition rate maybe relate to the bone cell proliferation. [ABSTRACT FROM AUTHOR]