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The development and application of a novel LC–MS/MS method for the measurement of Dolutegravir, Elvitegravir and Cobicistat in human plasma.
- Source :
-
Journal of Chromatography B: Analytical Technologies in the Biomedical & Life Sciences . Aug2016, Vol. 1027, p174-180. 7p. - Publication Year :
- 2016
-
Abstract
- Dolutegravir and Elvitegravir belongs to a class of integrase inhibitors which has recently been approved by the FDA for the treatment of HIV-infection. Elvitegravir and its co-administered booster drug, Cobicistat, has shown the potential to be a candidate for a one pill once a day regimen and is currently a component of many clinical trials. A sensitive LC–MS/MS method has been developed and validated for the simultaneous determination of these three drugs in human plasma. A liquid- liquid extraction was used as a sample preparation technique using 100 μL of plasma. The method was validated from 10 to 4000 ng/mL for Dolutegravir, Elvitegravir and Cobicistat. Chromatography was performed on XBridge C 18 2.1 mm × 50 mm column, using an 80:20 methanol/water mobile phase containing 0.1% formic acid on a gradient program. This method was successfully applied for ongoing clinical trials. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 15700232
- Volume :
- 1027
- Database :
- Academic Search Index
- Journal :
- Journal of Chromatography B: Analytical Technologies in the Biomedical & Life Sciences
- Publication Type :
- Academic Journal
- Accession number :
- 116406034
- Full Text :
- https://doi.org/10.1016/j.jchromb.2016.05.040