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Development and Validation of a Sensitive and Specific LC-MS-MS Method for the Determination of Acotiamide in Rat Plasma.
- Source :
-
Journal of Chromatographic Science . Jul2016, Vol. 54 Issue 6, p1004-1009. 6p. - Publication Year :
- 2016
-
Abstract
- Acotiamide is a new prokinetic drug that is used to treat functional dyspepsia (FD). A sensitive and specific LC-MS-MS method has been developed and validated for the analysis of acotiamide in rat plasma. The assay involved a simple protein precipitation (PPT) step with methanol-acetonitrile (50:50, v/v) and a gradient elution using a mobile phase consisting of water containing 0.1% formic acid and methanol containing 0.1% formic acid. The analytes were chromatographed on a reversephase Agilent Zorbax XDB C18 column (2.1 mm× 50 mm, 3.5 μm) with a flowrate of 0.50 mL/min. The analytes were monitored by tandem-mass spectrometry with positive electrospray ionization. The precursor-product transitions (m/z) in the positive ion mode were 451.4→271.3 and 386.2→122.2 for acotiamide and buspirone (internal standard, IS), respectively. The assay was shown to be linear over the range of 0.10-200 ng/mL, with a lower limit of quantification of 0.10 ng/mL. The method was shown to be reproducible and reliable with the inter- and intra-batch accuracy and precision were within ±15%. The assay has been successfully used for pharmacokinetic evaluation of acotiamide after intravenous and oral administration of 10 mg/kg acotiamide in rats. The oral absolute bioavailability (F) of acotiamide in rats was estimated to be 38.4 ± 13.5% with an elimination half-life (t1/2) value of 9.11 ± 0.40 h. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00219665
- Volume :
- 54
- Issue :
- 6
- Database :
- Academic Search Index
- Journal :
- Journal of Chromatographic Science
- Publication Type :
- Academic Journal
- Accession number :
- 116119045
- Full Text :
- https://doi.org/10.1093/chromsci/bmw035