成骨细胞介电特性检测的方法研究.

Objective:To establish a complete set of dielectric properties detective method for osteoblast by using mouse osteoblast. Methods: Different shape and size of cell dielectric spectroscopy detection device(dielectric cell) was designed and made, and the biocompatibility, including the toxicity test of leach liquor, HE staining was measured to observe the cell morphology. The cell were cultured in the measuring vessel, parallel conductance Gp and parallel capacitance Cp in 20Hz~10MHz was detected by impedance analyzer and draw the dielectric spectra. Results: Biocompatibility testing showed that the dielectric cell had no toxicity to cell and it can be used. Dielectric properties detection results showed that with the increase of frequency, the capacitance decreased, conductance slightly increased and then decreased. With the growth of the culture time, the Cp and Gp values all decreased. Moderate intensity magnetic field treated showed that the Cp and Gp values all declined. Conclusions: This method can provide a research bases for other dielectric property detective of adherent cells, and it can also provide a reference for adherent cells analysis based on this method. [ABSTRACT FROM AUTHOR]