Immunostimulating and Gram-negative-specific antibacterial cyclotides from the butterfly pea ( Clitoria ternatea).

Cyclotides are plant-derived, cyclic miniproteins with three interlocking disulfide bonds that have attracted great interests because of their excellent stability and potential as peptide therapeutics. In this study, we characterize the cyclotides of the medicinal plant Clitoria ternatea (butterfly pea) and investigate their biological activities. Using a combined proteomic and transcriptomic method, we identified 41 novel cyclotide sequences, which we named cliotides, making C. ternatea one of the richest cyclotide-producing plants to date. Selected members of the cationic cliotides display potent antibacterial activity specifically against Gram-negative bacteria with minimal inhibitory concentrations as low as 0.5 μ m. Remarkably, they also possess prominent immunostimulating activity. At a concentration of 1 μ m, cationic cliotides are capable of augmenting the secretion of various cytokines and chemokines in human monocytes at both resting and lipopolysaccharide-stimulated states. Chemokines such as macrophage inflammatory proteins 1α and 1β, interferon γ-induced protein 10, interleukin 8 and tumor necrosis factor α were among the most upregulated with up to 129-fold increase in secretion level. These findings suggest cyclotides can serve as potential candidates for novel immunomodulating therapeutics. Database The protein sequences reported in this paper (cT13-cT21) are available in the UniProt Knowledgebase under the accession numbers , , , , , , , and , respectively. The transcriptome data in this paper are available at the Sequence Read Archive database ( NCBI) under accession number . The protein precursors reported in this paper (ctc13, ctc15, ctc17-ctc19, ctc21-ctc53) are available at GenBank under the accession numbers , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , and , respectively. [ABSTRACT FROM AUTHOR]