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Pomegranate inhibits neuroinflammation and amyloidogenesis in IL-1β-stimulated SK-N-SH cells.
- Source :
-
European Journal of Nutrition . Jun2016, Vol. 55 Issue 4, p1653-1660. 8p. 8 Graphs. - Publication Year :
- 2016
-
Abstract
- Purpose: Pomegranate fruit, Punica granatum L. (Punicaceae), and its constituents have been shown to inhibit inflammation. In this study, we aimed to assess the effects of freeze-dried pomegranate (PWE) on PGE production in IL-1β-stimulated SK-N-SH cells. Methods: An enzyme immunoassay (EIA) was used to measure prostaglandin E (PGE) production from supernatants of IL-1β-stimulated SK-N-SH cells. Expression of COX-2, phospho-IκB, and phospho-IKK proteins was evaluated, while NF-κB reporter gene assay was carried out in TNFα-stimulated HEK293 cells to determine the effect of PWE on NF-κB transactivation. Levels of BACE-1 and Aβ in SK-N-SH cells stimulated with IL-1β were measured with an in cell ELISA. Results: PWE (25-200 μg/ml) dose dependently reduced COX-2-dependent PGE production in SK-N-SH cells stimulated with IL-1β. Phosphorylation of IκB and IKK was significantly ( p < 0.001) inhibited by PWE (50-200 μg/ml). Our studies also show that PWE (50-200 μg/ml) significantly ( p < 0.01) inhibited NF-κB transactivation in TNFα-stimulated HEK293 cells. Furthermore, PWE inhibited BACE-1 and Aβ expression in SK-N-SH cells treated with IL-1β. Conclusions: Taken together, our study demonstrates that pomegranate inhibits inflammation, as well as amyloidogenesis in IL-1β-stimulated SK-N-SH cells. We propose that pomegranate is a potential nutritional strategy in slowing the progression of neurodegenerative disorders such as Alzheimer's disease. [ABSTRACT FROM AUTHOR]
- Subjects :
- *PROTEIN analysis
*GENES
*AMYLOIDOSIS
*ANTI-inflammatory agents
*BIOLOGICAL assay
*BIOLOGICAL models
*CELL physiology
*DOSE-response relationship in biochemistry
*ENZYME-linked immunosorbent assay
*IMMUNOASSAY
*NEURONS
*PHOSPHORYLATION
*POMEGRANATE
*PROBABILITY theory
*PROSTAGLANDINS
*RESEARCH funding
*STATISTICS
*T-test (Statistics)
*DNA-binding proteins
*DATA analysis
*STATISTICAL significance
*DESCRIPTIVE statistics
*IN vitro studies
*ONE-way analysis of variance
*PHARMACODYNAMICS
*PREVENTION
Subjects
Details
- Language :
- English
- ISSN :
- 14366207
- Volume :
- 55
- Issue :
- 4
- Database :
- Academic Search Index
- Journal :
- European Journal of Nutrition
- Publication Type :
- Academic Journal
- Accession number :
- 115529566
- Full Text :
- https://doi.org/10.1007/s00394-015-0984-0