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Specific determination of myo-inositol in multivitamin pharmaceutical preparations by a flow injection system using a myo-inositol dehydrogenase reactor coupled with a glucose eliminating enzyme reactor
- Source :
-
Journal of Pharmaceutical & Biomedical Analysis . Dec2003, Vol. 33 Issue 5, p1175. 6p. - Publication Year :
- 2003
-
Abstract
- A flow injection system for myo-inositol determination in multivitamin pharmaceutical preparations using two enzyme reactors was developed. Myo-inositol was detected using a fluorophotometer, to measure the fluorescence of NADH produced from NAD+ by a myo-inositol dehydrogenase reactor (IDR) containing myo-inositol dehydrogenase immobilized on porous glass. Enhanced interference due to excess glucose included in a multivitamin pharmaceutical preparation as a sweetener was eliminated by a glucose eliminating reactor (GER) co-immobilized with three enzymes (glucose oxidase, mutarotase and catalase). The calibration coefficient for the standard curve was 0.9993 for myo-inositol detection in the range of 1–5 μg/ml. Myo-inositol was determined even in the presence of glucose concentrations of 140–420 μg/ml. The recovery of myo-inositol added to the multivitamin pharmaceutical preparation was 99.6% (n=9). [Copyright &y& Elsevier]
- Subjects :
- *FLOW injection analysis
*ENZYMES
*FLUORESCENCE
*POROUS materials
Subjects
Details
- Language :
- English
- ISSN :
- 07317085
- Volume :
- 33
- Issue :
- 5
- Database :
- Academic Search Index
- Journal :
- Journal of Pharmaceutical & Biomedical Analysis
- Publication Type :
- Academic Journal
- Accession number :
- 11538304
- Full Text :
- https://doi.org/10.1016/S0731-7085(03)00427-8