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Simultaneous separation and determination of active components in Cordyceps sinensis and Cordyceps militarris by LC/ESI-MS
- Source :
-
Journal of Pharmaceutical & Biomedical Analysis . Dec2003, Vol. 33 Issue 5, p1155. 8p. - Publication Year :
- 2003
-
Abstract
- A simple and rapid isocratic LC/MS coupled with electrospray ionization (ESI) method for simultaneous separation and determination of adenine, hypoxanthine, adenosine and cordycepin in Cordyceps sinensis (Cs) and its substitutes was developed. 2-Chloroadenosine was used as internal standard for this assay. The optimum separation for these analytes was achieved using the mixture of water, methanol and formic acid (85:14:1, v/v/v) as a mobile phase and a 2.0×150 mm Shimadzu VP-ODS column. Selective ion monitoring (SIM) mode ([M+H]+ at m/z 136, 137, 268, 252 and 302) was used for quantitative analysis of above four active components. The regression equations were liner in the range of 1.4–140.0 μg ml−1 for adenine, 0.6–117.5 μg ml−1 for hypoxanthine, 0.5–128.5 μg ml−1 for adenosine and 0.5–131.5 μg ml−1 for cordycepin. The limits of quantitation (LOQ) and detection (LOD) were, respectively 1.4 and 0.5 μg ml−1 for adenine, 0.6 and 0.2 μg ml−1 for hypoxanthine, 0.5 and 0.1 μg ml−1 for adenosine and cordycepin. The recoveries of four constituents were from 93.5 to 107.0%. The nucleoside contents of various types of natural Cs and its substitutes were determined and compared with this developed method. [Copyright &y& Elsevier]
- Subjects :
- *ELECTROSPRAY ionization mass spectrometry
*ADENINE
*ADENOSINES
Subjects
Details
- Language :
- English
- ISSN :
- 07317085
- Volume :
- 33
- Issue :
- 5
- Database :
- Academic Search Index
- Journal :
- Journal of Pharmaceutical & Biomedical Analysis
- Publication Type :
- Academic Journal
- Accession number :
- 11538302
- Full Text :
- https://doi.org/10.1016/S0731-7085(03)00415-1