Back to Search
Start Over
Prevalence of human papillomavirus types and variants and p16INK4a expression in head and neck squamous cells carcinomas in São Paulo, Brazil.
- Source :
-
Infectious Agents & Cancer . 5/4/2016, Vol. 11, p1-7. 7p. 4 Charts, 1 Graph. - Publication Year :
- 2016
-
Abstract
- Background: Human papillomavirus (HPV) prevalence in head and neck squamous cell carcinomas (HNSCC) diverges geographically. The reliability of using p16INK4a expression as a marker of viral infection is controversial in HNSCC. We evaluated HPV types and HPV-16 variants prevalence, and p16INK4a expression in HNSCC specimens provided by two different Institutions in São Paulo. Methods: HPV DNA from formalin-fixed specimens was accessed by Inno-LiPA, HPV-16 variants by PCR-sequencing, and p16INK4a protein levels by immunohistochemistry. Results: Overall, HPV DNA was detected among 19.4 % of the specimens (36/186). Viral prevalence was higher in the oral cavity (25.0 %, 23/92) then in other anatomical sites (oropharynx 14,3 %, larynx 13.7 %) when samples from both Institutions were analyzed together. HPV prevalence was also higher in the oral cavity when samples from both Institutions were analyzed separately. HPV-16 was the most prevalent type identified in 69.5 % of the HPV positive smaples and specimens were assigned into Asian-American (57.2 %) or European (42.8 %) phylogenetic branches. High expression of p16INK4a was more common among HPV positive tumors. Conclusion: Our results support a role for HPV-16 in a subset of HNSCC. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 17509378
- Volume :
- 11
- Database :
- Academic Search Index
- Journal :
- Infectious Agents & Cancer
- Publication Type :
- Academic Journal
- Accession number :
- 115283927
- Full Text :
- https://doi.org/10.1186/s13027-016-0067-8