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Direct analysis of [6,6-2H2]glucose and [U-13C6]glucose dry blood spot enrichments by LC–MS/MS.
- Source :
-
Journal of Chromatography B: Analytical Technologies in the Biomedical & Life Sciences . Jun2016, Vol. 1022, p242-248. 7p. - Publication Year :
- 2016
-
Abstract
- A liquid chromatography tandem mass spectrometry (LC–MS/MS) using multiple reaction monitoring (MRM) in a triple-quadrupole scan mode was developed and comprehensively validated for the determination of [6,6- 2 H 2 ]glucose and [U- 13 C 6 ]glucose enrichments from dried blood spots (DBS) without prior derivatization. The method is demonstrated with dried blood spots obtained from rats administered with a primed-constant infusion of [U- 13 C 6 ]glucose and an oral glucose load enriched with [6,6- 2 H 2 ]glucose. The sensitivity is sufficient for analysis of the equivalent to <5 μL of blood and the overall method was accurate and precise for the determination of DBS isotopic enrichments. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 15700232
- Volume :
- 1022
- Database :
- Academic Search Index
- Journal :
- Journal of Chromatography B: Analytical Technologies in the Biomedical & Life Sciences
- Publication Type :
- Academic Journal
- Accession number :
- 115244850
- Full Text :
- https://doi.org/10.1016/j.jchromb.2016.04.027