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The jasmonate-responsive Aa MYC2 transcription factor positively regulates artemisinin biosynthesis in Artemisia annua.

Authors :
Shen, Qian
Lu, Xu
Yan, Tingxiang
Fu, Xueqing
Lv, Zongyou
Zhang, Fangyuan
Pan, Qifang
Wang, Guofeng
Sun, Xiaofen
Tang, Kexuan
Source :
New Phytologist. Jun2016, Vol. 210 Issue 4, p1269-1281. 13p.
Publication Year :
2016

Abstract

The plant Artemisia annua is well known due to the production of artemisinin, a sesquiterpene lactone that is widely used in malaria treatment. Phytohormones play important roles in plant secondary metabolism, such as jasmonic acid ( JA), which can induce artemisinin biosynthesis in A. annua. Nevertheless, the JA-inducing mechanism remains poorly understood., The expression of gene Aa MYC2 was rapidly induced by JA and Aa MYC2 binds the G-box-like motifs within the promoters of gene CYP71 AV1 and DBR2, which are key structural genes in the artemisinin biosynthetic pathway., Overexpression of Aa MYC2 in A. annua significantly activated the transcript levels of CYP71 AV1 and DBR2, which resulted in an increased artemisinin content. By contrast, artemisinin content was reduced in the RNAi transgenic A. annua plants in which the expression of Aa MYC2 was suppressed. Meanwhile, the RNAi transgenic A. annua plants showed lower sensitivity to methyl jasmonate treatment than the wild-type plants., These results demonstrate that Aa MYC2 is a positive regulator of artemisinin biosynthesis and is of great value in genetic engineering of A. annua for increased artemisinin production. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
0028646X
Volume :
210
Issue :
4
Database :
Academic Search Index
Journal :
New Phytologist
Publication Type :
Academic Journal
Accession number :
115230822
Full Text :
https://doi.org/10.1111/nph.13874