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The Impact of Serum Amyloid P-Component on Gene Expression in RAW264.7 Mouse Macrophages.
- Source :
-
BioMed Research International . 4/28/2016, Vol. 2016, p1-9. 9p. - Publication Year :
- 2016
-
Abstract
- Serum amyloid P-component (SAP) contributes to host defense and prevents fibrosis. Macrophages are the most abundant inflammatory cell type in atherosclerotic plaques. In the present study, using 3H-cholesterol-labeled counting radioactivity assay, we demonstrated that the apoAI-mediated cholesterol efflux in RAW264.7 macrophages was increased by SAP treatment in a time- and dose-dependent manner. We analyzed global gene expression changes upon SAP treatment using RNA sequencing. As a result, a total of 175 differentially expressed genes were identified, of which 134 genes were downregulated and 41 genes were upregulated in SAP treated cells compared to control cells. Quantitative RT-PCR analysis confirmed decreased expression of 5 genes and an increase in expression of 1 gene upon SAP treatment. Gene ontology analysis showed that genes involved in response to stimulus were significantly enriched in differentially expressed genes. Beyond protein-coding genes, we also identified 8 differentially expressed long noncoding RNAs. Our study may provide new insights into mechanisms underlying the functional role of SAP in macrophages. [ABSTRACT FROM AUTHOR]
- Subjects :
- *MACROPHAGES
*RNA analysis
*ANALYTICAL biochemistry
*ANIMAL experimentation
*ATHEROSCLEROSIS
*BIOLOGICAL assay
*COLLECTION & preservation of biological specimens
*CELL culture
*CLUSTER analysis (Statistics)
*DOSE-response relationship in biochemistry
*GENE expression
*GLYCOPROTEINS
*MICE
*POLYMERASE chain reaction
*PROBABILITY theory
*RADIOACTIVITY
*RESEARCH funding
*T-test (Statistics)
*REVERSE transcriptase polymerase chain reaction
*DATA analysis software
*DESCRIPTIVE statistics
*SEQUENCE analysis
*PHYSIOLOGY
Subjects
Details
- Language :
- English
- ISSN :
- 23146133
- Volume :
- 2016
- Database :
- Academic Search Index
- Journal :
- BioMed Research International
- Publication Type :
- Academic Journal
- Accession number :
- 114918349
- Full Text :
- https://doi.org/10.1155/2016/9380290