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miR-145a-5p Promotes Myoblast Differentiation.
- Source :
-
BioMed Research International . 4/28/2016, Vol. 2016, p1-10. 10p. - Publication Year :
- 2016
-
Abstract
- MicroRNAs are a class of 18–22-nucleotide noncoding RNAs that posttranscriptionally regulate gene expression and have been shown to play an important role during myoblast differentiation. In this study, we found that the expression of miR-145a-5p was gradually increased during C2C12 myoblast differentiation, and miR-145a-5p inhibitors or mimics significantly suppressed or promoted the relative expression of specific myogenesis related marker genes. Moreover, overexpression or inhibition of miR-145a-5p enhanced or repressed the expression of some special genes involved in the endogenous Wnt signaling pathway during C2C12 myoblast differentiation, including Wnt5a, LRP5, Axin2, and β-catenin. These results indicated that miR-145a-5p might be considered as a new myogenic differentiation-associated microRNA that can promote C2C12 myoblast differentiation by enhancing genes related to myoblasts differentiation. [ABSTRACT FROM AUTHOR]
- Subjects :
- *STEM cells
*RNA physiology
*CELL culture
*CELL differentiation
*CELL lines
*CELLULAR signal transduction
*GENE expression
*GENETIC research
*IMMUNOHISTOCHEMISTRY
*MICE
*POLYMERASE chain reaction
*PROBABILITY theory
*RESEARCH funding
*T-test (Statistics)
*TRANSCRIPTION factors
*GENETIC markers
*DATA analysis software
*DESCRIPTIVE statistics
*SEQUENCE analysis
*ONE-way analysis of variance
*PHYSIOLOGY
Subjects
Details
- Language :
- English
- ISSN :
- 23146133
- Volume :
- 2016
- Database :
- Academic Search Index
- Journal :
- BioMed Research International
- Publication Type :
- Academic Journal
- Accession number :
- 114918345
- Full Text :
- https://doi.org/10.1155/2016/5276271