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Canstatin-N fragment inhibits in vitro endothelial cell proliferation and suppresses in vivo tumor growth
- Source :
-
Biochemical & Biophysical Research Communications . Dec2003, Vol. 312 Issue 3, p801. 5p. - Publication Year :
- 2003
-
Abstract
- Type IV collagen is one of the components of vascular basement involved in regulation of angiogenesis. Canstatin, the non-collagenous 1 (NC1) domain of α2 chain of type IV collagen, was identified as an inhibitor of angiogenesis and tumor growth by Kamphaus et al. Our previous studies showed that canstatin-N, the N-terminal 1–89 amino acid fragment of canstatin, inhibited the neovascularization in a dose-dependent manner as tested by CAM assay. In the present study, we demonstrate that canstatin-N produced in Escherichia coli specifically inhibited in vitro the proliferation of human umbilical vein endothelial cells (ECV304) and significantly induced apoptosis. The apoptosis-inducing activity of canstatin-N was much stronger than that of canstatin, indicating that the apoptosis-inducing activity of canstatin is likely located within its N-terminal 1–89 amino acid fragment. Canstatin-N also suppressed in vivo growth of B16 murine melanoma in BALB/c mice at a dosage of 10 mg/kg/day. These results suggest that canstatin-N is a useful candidate molecule for inhibition of tumor growth. [Copyright &y& Elsevier]
- Subjects :
- *COLLAGEN
*ANTINEOPLASTIC agents
*NEOVASCULARIZATION
*ENDOTHELINS
Subjects
Details
- Language :
- English
- ISSN :
- 0006291X
- Volume :
- 312
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Biochemical & Biophysical Research Communications
- Publication Type :
- Academic Journal
- Accession number :
- 11469310
- Full Text :
- https://doi.org/10.1016/j.bbrc.2003.11.003