In vitro Evaluation of Active Phytochemicals from Tabernaemontana alternifolia (Roxb) Root Against the Naja naja and Echis carinatus Indian Snake Venom.

The different extracts ofTabernaemontana alternifoliaroots were used to evaluate the inhibitions of protease, 5’ nucleotidase, phosphomonoesterase, phosphodiesterase, acetylcholinesterase, phospholipase A2, hyaluronidase enzymes present in snake venom. All extracts were found to inhibit acetylcholinesterase and 5’nucleotidase in both snakes’ venom. The methanolic extract inhibited phosphomonoesterase, phosphodiesterase, hyaluronidase and phospholipase A2in both venom and protease in Cobra venom. As methanolic extract inhibited maximum number of toxic enzymes it was considered as an active extract. The study would be further validated byin vivoand pharmacological studies. [ABSTRACT FROM AUTHOR]