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Simultaneous Determination of Diosmin and Hesperidin in Pharmaceuticals by RPLC using Ionic Liquids as Mobile Phase Modifiers.
- Source :
-
Iranian Journal of Pharmaceutical Research . Winter2016, Vol. 15 Issue 1, p141-148. 8p. - Publication Year :
- 2016
-
Abstract
- Diosmin and hesperidin are natural flavonoid glycosides found in various plant materials, mainly in citrus fruits in different concentrations. Diosmin for pharmaceutical use is obtained mainly semi-synthetically from hesperidin. Hesperidin often accompanies diosmin as a natural impurity in different pharmaceutical formulations; therefore, a simple, fast and precise method for the simultaneous assay of diosmin and hesperidin in pharmaceutical formulations has been developed to control their contents. Chromatographic resolution was performed using a column with C-18 packing and the following mobile phase: methanol/water (45:55, v/v) with 0.025% added didecyldimethylammonium lactate, which significantly affects retention, shortening analysis time and having a positive impact on the symmetry of resulting chromatographic peaks. The method shows linearity between 2.5 and 100 μg/mL, high repeatability (0.39 and 0.42% for diosmin and hesperidin, respectively) and accuracy of 96 to 102% for both the assayed compounds. Intraday and interday precision of the new method were less than RSD% 1, 2. The limit of detection of the assayed compounds is 2.5 and 1.2 gg/mL for diosmin and hesperidin, respectively. The method was tested on several pharmaceutical products available in Poland. [ABSTRACT FROM AUTHOR]
- Subjects :
- *DIOSMIN
*HESPERIDIN
*FLAVONOID glycosides
*IONIC liquids
*CHROMATOGRAPHIC analysis
Subjects
Details
- Language :
- English
- ISSN :
- 17350328
- Volume :
- 15
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Iranian Journal of Pharmaceutical Research
- Publication Type :
- Academic Journal
- Accession number :
- 114137717