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Mapping regions in Ste5 that support Msn5-dependent and -independent nuclear export.
- Source :
-
Biochemistry & Cell Biology . 2016, Vol. 94 Issue 2, p109-128. 20p. - Publication Year :
- 2016
-
Abstract
- Careful control of the available pool of the MAPK scaffold Ste5 is important for mating-pathway activation and the prevention of inappropriate mating differentiation in haploid Saccharomyces cerevisiae. Ste5 shuttles constitutively through the nucleus, where it is degraded by a ubiquitin-dependent mechanism triggered by G1 CDK phosphorylation. Here we narrow-down regions of Ste5 that mediate nuclear export. Four regions in Ste5 relocalize SV40-TAgNLS-GFP-GFP from nucleus to cytoplasm. One region is N-terminal, dependent on exportin Msn5/Ste21/Kap142, and interacts with Msn5 in 2 hybrid assays independently of mating pheromone, Fus3, Kss1, Ptc1, the NLS/PM, and RING-H2. A second region overlaps the PH domain and Ste11 binding site and 2 others are on the vWA domain and include residues essential for MAPK activation. We find no evidence for dependence on Crm1/Xpo1, despite numerous potential nuclear export sequences (NESs) detected by LocNES and NetNES1.1 predictors. Thus, Msn5 (homolog of human Exportin-5) and one or more exportins or adaptor molecules besides Crm1/Xpo1 may regulate Ste5 through multiple recognition sites. [ABSTRACT FROM AUTHOR]
- Subjects :
- *PHEROMONE receptors
*HAPLOIDY
*SACCHAROMYCES cerevisiae
*PHOSPHORYLATION
*CYTOPLASM
Subjects
Details
- Language :
- English
- ISSN :
- 08298211
- Volume :
- 94
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Biochemistry & Cell Biology
- Publication Type :
- Academic Journal
- Accession number :
- 114013627
- Full Text :
- https://doi.org/10.1139/bcb-2015-0101