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IL-18 increases invasiveness of HL-60 myeloid leukemia cells: up-regulation of matrix metalloproteinases-9 (MMP-9) expression
- Source :
-
Leukemia Research . Jan2004, Vol. 28 Issue 1, p91. 5p. - Publication Year :
- 2004
-
Abstract
- Similar to matrix metalloproteinases (MMP-9/-2), IL-18 was overexpressed in some hematologic malignancies such as acute myeloid leukemia (AML), which is associated with a poor clinical outcome. To establish a possible functional relationship between IL-18 and MMPs in myeloid leukemia, we used semi-quantitative PCR and zymographic analysis to examine whether IL-18 stimulates human myeloid leukemia cell line HL-60 to produce MMPs and/or specific tissue inhibitors (TIMPs), and to degrade extracellular matrix (ECM) gel in vitro. In the ECM invasion assay IL-18 significantly up-regulated transmigration of HL-60 cells, which in turn was inhibited by a synthetic MMP inhibitor: O-phenanthroline (o-PE), anti-MMP-9, anti-MMP-2 as well as anti-IL-18 monoclonal antibody (McAb), respectively, suggesting that induction of gelatinases by IL-18 leads to ECM degradation by these cells. Moreover, IL-18 could significantly increase MMP-9 but not MMP-2 production at both mRNA and/or protein level, slightly up-regulate TIMP-1 mRNA, and clearly induce TIMP-2 mRNA secretion. We postulate that IL-18 may in part play a role in the clinical aggressiveness of human myeloid leukemia by stimulating MMP-9 production. [Copyright &y& Elsevier]
- Subjects :
- *METALLOPROTEINASES
*HEMATOLOGY
*ACUTE myeloid leukemia
Subjects
Details
- Language :
- English
- ISSN :
- 01452126
- Volume :
- 28
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Leukemia Research
- Publication Type :
- Academic Journal
- Accession number :
- 11400375
- Full Text :
- https://doi.org/10.1016/S0145-2126(03)00121-8