Implementation of a new integrated d-lactic acid biosensor in a semiautomatic FIA system for the simultaneous determination of lactic acid enantiomers. Application to the analysis of beer samples.

An integrated amperometric d -lactic acid biosensor involving a gold film deposited by sputtering on a stainless steel disk electrode where the enzymes d -lactic acid dehydrogenase (DLDH) and diaphorase (DP) as well as the redox mediator tetrathiafulvalene (TTF) are coimmobilized by using a dialysis membrane, is reported in this work. Amperometry in stirred solutions at a detection potential of +0.15 V (vs Ag/AgCl reference electrode) provided a linear calibration plot for d -lactic acid over the 1.0×10 −4 to 3.8×10 −3 g L −1 concentration range, with a limit of detection of 3.1×10 −5 g L −1 . The usefulness of the biosensor was demonstrated by determining d -lactic acid in beer samples with good results. Additionally, the biosensor was implemented together with a commercial l −lactic amperometric biosensor in a semiautomatic flow-injection analysis (FIA) system able to perform a rapid and simple stereo-specific determination of d - and d -lactic without a previous separation step. The operational characteristics of the biosensors under flow conditions were evaluated and its applicability was demonstrated through the simultaneous determination of both enantiomers in beer samples. [ABSTRACT FROM AUTHOR]