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Intravenous PEP-1-GDNF is protective after focal cerebral ischemia in rats.

Authors :
Liu, Yaning
Wang, Shangwu
Luo, Shijian
Li, Zhendong
Liang, Fengyin
Zhu, Yanan
Pei, Zhong
Huang, Ruxun
Source :
Neuroscience Letters. Mar2016, Vol. 617, p150-155. 6p.
Publication Year :
2016

Abstract

Glial cell line-derived neurotrophic factor (GDNF) is a potential therapeutic protein on a variety of central nervous system diseases including ischemic stroke. However, GDNF is a large molecule that cannot cross the blood–brain barrier (BBB), which is still intact in the early hours after stroke when neural rescue is possible. PEP-1 protein transduction domain can deliver protein cargo across the cell membrane and the BBB. In the present study, we generated a novel fusion protein PEP-1-GDNF and examined whether PEP-1-GDNF is protective in focal cerebral ischemia. PEP-1-GDNF (200 μg/kg) or PBS was intravenously applied over 5 min immediately after reperfusion of 90 min transient middle cerebral artery occlusion (MCAO). After 28 days, rats were deeply anesthetized and decapitated. Behavioral tests were performed during this period. The results showed that PEP-1-GDNF significantly reduced the infarct volume and improved behavioral function. Further, PEP-1-GDNF promoted the cell proliferation and differentiation in the dentate gyrus of the hippocampus and attenuated ischemia-induced learning and memory damage. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
03043940
Volume :
617
Database :
Academic Search Index
Journal :
Neuroscience Letters
Publication Type :
Academic Journal
Accession number :
113793384
Full Text :
https://doi.org/10.1016/j.neulet.2016.02.017