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Effect of platinum nanoparticles on cell death induced by ultrasound in human lymphoma U937 cells.

Authors :
Jawaid, Paras
Rehman, Mati Ur
Hassan, Mariame Ali
Zhao, Qing Li
Li, Peng
Miyamoto, Yusei
Misawa, Masaki
Ogawa, Ryohei
Shimizu, Tadamichi
Kondo, Takashi
Source :
Ultrasonics Sonochemistry. Jul2016, Vol. 31, p206-215. 10p.
Publication Year :
2016

Abstract

In this study, we report on the potential use of platinum nanoparticles (Pt-NPs), a superoxide dismutase (SOD)/catalase mimetic antioxidant, in combination with 1 MHz ultrasound (US) at an intensity of 0.4 W/cm 2 , 10% duty factor, 100 Hz PRF, for 2 min. Apoptosis induction was assessed by DNA fragmentation assay, cell cycle analysis and Annexin V-FITC/PI staining. Cell killing was confirmed by cell counting and microscopic examination. The mitochondrial and Ca 2+ -dependent pathways were investigated. Caspase-8 expression and autophagy-related proteins were detected by spectrophotometry and western blot analysis, respectively. Intracellular reactive oxygen species (ROS) elevation was detected by flow cytometry, while extracellular free radical formation was assessed by electron paramagnetic resonance spin trapping spectrometry. The results showed that Pt-NPs exerted differential effects depending on their internalization. Pt-NPs functioned as potent free radical scavengers when added immediately before sonication while pre-treatment with Pt-NPs suppressed the induction of apoptosis as well as autophagy (AP), and resulted in enhanced cell killing. Dead cells displayed the features of pyknosis. The exact mode of cell death is still unclear. In conclusion, the results indicate that US-induced AP may contribute to cell survival post sonication. To our knowledge this is the first study to discuss autophagy as a pro-survival pathway in the context of US. The combination of Pt-NPs and US might be effective in cancer eradication. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
13504177
Volume :
31
Database :
Academic Search Index
Journal :
Ultrasonics Sonochemistry
Publication Type :
Academic Journal
Accession number :
113581643
Full Text :
https://doi.org/10.1016/j.ultsonch.2015.12.013