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The cell-permeable Aβ1-6A2VTAT(D) peptide reverts synaptopathy induced by Aβ1-42wt.

Authors :
Cimini, Sara
Sclip, Alessandra
Mancini, Simona
Colombo, Laura
Messa, Massimo
Cagnotto, Alfredo
Di Fede, Giuseppe
Tagliavini, Fabrizio
Salmona, Mario
Borsello, Tiziana
Source :
Neurobiology of Disease. May2016, Vol. 89, p101-111. 11p.
Publication Year :
2016

Abstract

Alzheimer disease (AD) is the most prevalent form of dementia. Loss of hippocampal synapses is the first neurodegenerative event in AD. Synaptic loss has been associated with the accumulation in the brain parenchyma of soluble oligomeric forms of amyloid β peptide (Aβ1-42 wt ). Clinical observations have shown that a mutation in the APP protein (A673V) causes an early onset AD-type dementia in homozygous carriers while heterozygous carriers are unaffected. This mutation leads to the formation of mutated Aβ peptides (Aβ1-42 A2V ) in homozygous patients, while in heterozygous subjects both Aβ1-42 wt and Aβ1-42 A2V are present. To better understand the impact of the A673V mutation in AD, we analyzed the synaptotoxic effect of oligomers formed by aggregation of different Aβ peptides (Aβ1-42 wt or Aβ1-42 A2V ) and the combination of the two Aβ1-42 MIX (Aβ1-42 wt and Aβ1-42 A2V ) in an in vitro model of synaptic injury. We showed that Aβ1-42 A2V oligomers are more toxic than Aβ1-42 wt oligomers in hippocampal neurons, confirming the results previously obtained in cell lines. Furthermore, we reported that oligomers obtained by the combination of both wild type and mutated peptides (Aβ1-42 MIX ) did not exert synaptic toxicity. We concluded that the combination of Aβ1-42 wt and Aβ1-42 A2V peptides hinders the toxicity of Aβ1-42 A2V and counteracts the manifestation of synaptopathy in vitro. Finally we took advantage of this finding to generate a cell-permeable peptide for clinical application, by fusing the first six residues of the Aβ1-42 A2V to the TAT cargo sequence (Aβ1-6 A2V TAT(D)). Noteworthy, the treatment with Aβ1-6 A2V TAT(D) confers neuroprotection against both in vitro and in vivo synaptopathy models. Therefore Aβ1-6 A2V TAT(D) may represent an innovative therapeutic tool to prevent synaptic degeneration in AD. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
09699961
Volume :
89
Database :
Academic Search Index
Journal :
Neurobiology of Disease
Publication Type :
Academic Journal
Accession number :
113541771
Full Text :
https://doi.org/10.1016/j.nbd.2015.12.013