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MicroRNA-9 controls apoptosis of neurons by targeting monocyte chemotactic protein-induced protein 1 expression in rat acute spinal cord injury model.
- Source :
-
Brain Research Bulletin . Mar2016, Vol. 121, p233-240. 8p. - Publication Year :
- 2016
-
Abstract
- Objective For the purpose of an early identification of intervention targets for acute spinal cord injury (ASCI), we investigated the changes in expression levels of microRNA-9 (miR-9) and MCPIP1 in rat ASCI model. Method A total of 108 healthy rats were randomly divided into non-ASCI group ( n = 18) and five ASCI groups, 6 h, 12 h, 24 h, 3 days and 7 days, representing the experimental time points following ASCI ( n = 18 per group). Hematoxylin and eosin (HE) staining was used to assess the ASCI damage, and quantitative real-time PCR (qRT-PCR) and in situ hybridization (ISH) were employed for the detection of miR-9 and MCPIP1 mRNA expression. Results HE staining results showed normal neuronal morphology in the non-ASCI group, but spinal cord tissue at 6 h after ASCI showed developing neuronal necrosis. Acute inflammatory response was evident at 12 h and 24 h, with immune cells infiltrating into the gray matter. Vascular permeability increased and the nerve cells in gray-white matter exhibited extensive damage and necrosis at 24 h and 7 days after ASCI. MiR-9 expression in ASCI tissue was significantly lower than that in normal spinal cord tissue. Statistical analysis showed a significant decrease in miR-9 expression in all the ASCI groups, compared to the non-ASCI group. Results from real-time PCR analysis revealed that MCPIP1 expression in all the ASCI groups was significantly higher than the non-ASCI group, and MCPIP1 expressions gradually increased with times at 6h–24 h after ASCI. ISH revealed the following results after ASCI (1) miR-9 and MCPIP1 mRNA expression mainly distributed in ventral horn motor neurons, (2) miR-9 expression was high at 7 day after ASCI and (3) in the non-ASCI group, MCPIP1 expression was high at 6 h, 12 h, 24 h and 3 days. Conclusion MCPIP1 is significantly up-regulated after ASCI. The negative relationship between MCPIP1 and miR-9 suggest that MCPIP1 mRNA could be a target of miR-9 during ASCI. Thus, miR-9 is a marker for apoptosis in neurons, and an excellent therapeutic target for ASCI patients. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 03619230
- Volume :
- 121
- Database :
- Academic Search Index
- Journal :
- Brain Research Bulletin
- Publication Type :
- Academic Journal
- Accession number :
- 113539578
- Full Text :
- https://doi.org/10.1016/j.brainresbull.2016.01.011