Spherical silver nanoparticles in the detection of thermally denatured collagens.

We have developed a rapid colorimetric method to determine the concentration of denatured collagen in solution, which is based on the collagen-silver nanoparticle corona formation. Using the proposed method, the lowest detectable concentration of denatured collagen protein in a solution of pure collagen was 14.7, 8.5, and 8.6 μg mL for porcine (PCOL), rat tail (RCOL), and type I human recombinant (HCOL) collagen, respectively. [Figure not available: see fulltext.] [ABSTRACT FROM AUTHOR]