阻断钙激活钾通道对宫颈癌细胞增殖及钾通道电流的影响.

Objective To investigate the effects of blocking intermediate-conductance-Ca2+-activated K+ channels ( IKCa1) on the cell proliferation and the membrane potential changes of IKCa1 in cervical cancer HeLa cells. Methods IKCal of HeLa cells was blocked with IKCa1 channel inhibitor (clotrimazole, CLT) and RNA interference, respectively. RT-PCR was used to detect the expression of IKCal mRNA in HeLa cells. The OD value of cells was detected by MTT, and IKCal current was detected by the patch clamp technique in HeLa cells. Results When IKCal was blocked with CLT in HeLa cells, the expression of IKCal mRNA was decreased, IKCal current wakened and the OD value of cells decreased as compared with that of the control group (all P<0. 05). When IKCal was blocked by small interfering RNA technology in HeLa cells, the expression of IKCal mRNA reduced, IKCal current wakened, and the OD value of cells decreased as com-pared with that of the blank group and negative transfection group (all P<0. 05). Conclusions Blocking IKCa1 could inhibit cell proliferation, down-regualte the expression of IKCa1 mRNA and cut down the IKCal current in HeLa cells. IKCa1 could regulate the cell proliferation of HeLa cells by directly regulating the membrane potential and thus affecting its signal transmission. [ABSTRACT FROM AUTHOR]