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Identification and molecular characterization of border disease virus (BDV) from sheep in India.

Authors :
Mishra, Niranjan
Rajukumar, Katherukamem
Vilcek, Stefan
Kalaiyarasu, Semmannan
Behera, Sthita Pragnya
Dubey, Pooja
Nema, Ram Kumar
Gavade, Vishal Balbhim
Dubey, Shiv Chandra
Kulkarni, D.D.
Source :
Comparative Immunology, Microbiology & Infectious Diseases. Feb2016, Vol. 44, p1-7. 7p.
Publication Year :
2016

Abstract

Pestiviruses isolated from sheep and goats in India thus far have been bovine viral diarrhoea virus 1 (BVDV-1) or BVDV-2. During routine genetic typing of pestiviruses in the years 2009-10, border disease virus (BDV) was detected in eight Indian sheep of a flock showing clinical signs of BD by real time RT-PCR. All the samples yielded positive virus isolates in cell culture but were found negative by a BVDV antigen ELISA. A representative BDV isolate was characterized at genetic and antigenic level. Phylogenetic analysis carried out in 5′-UTR, N pro and E2 regions of genome typed the Indian BDV isolate as BDV-3. A more detailed analysis in N pro and entire region coding structural proteins showed that the N pro (168), C (100 aa), E rns (227 aa), E1 (195 aa) and E2 (373 aa) proteins were of size characteristic for BDV reference strain X818. Antigenic differences were evident between the BDV-3 isolate and previously reported BDV-1, BDV-5 and BDV-7 strains. Although origin of BDV-3 in India is not clear, the results reflect probable introduction through trade in sheep between India and other countries or BDV-3 may be more widely distributed. Additionally, this study suggests that for diagnosis of BDV infection, the commercial BVDV Ag-ELISA should be used with caution. This is the first identification of BDV in sheep in India which highlights the need for continued pestivirus surveillance and assessing its impact on sheep and goat production. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
01479571
Volume :
44
Database :
Academic Search Index
Journal :
Comparative Immunology, Microbiology & Infectious Diseases
Publication Type :
Academic Journal
Accession number :
112744288
Full Text :
https://doi.org/10.1016/j.cimid.2015.11.001