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Enantioselective analysis of etodolac in human plasma by LC–MS/MS: Application to clinical pharmacokinetics.
- Source :
-
Journal of Pharmaceutical & Biomedical Analysis . Feb2016, Vol. 120, p120-126. 7p. - Publication Year :
- 2016
-
Abstract
- Etodolac is a non-steroidal anti-inflammatory drug with preferential inhibition of cyclooxigenase-2 and is widely used in the management of pain in patients with inflammatory arthritis. Etodolac is available as a racemic mixture of (−)-( R )-Etodolac and (+)-( S )-Etodolac; cyclooxigenases inhibition is attributed to (+)-( S )-Etodolac. According to our knowledge, this is the first method for determination of etodolac enantiomers in plasma using LC–MS/MS. Plasma extraction were performed with 25 μL of plasma and 1 mL of n-hexane:ethyl acetate (95:5); racemic ibuprofen was used as internal standard. Resolution of enantiomers were performed in a Chiralcel ® OD-H column; deprotonated [M-H] − and their respective ion products were monitored at transitions of 286 > 242 for etodolac enantiomers and 205 > 161 for ibuprofen. The quantitation limit was 3.2 ng/mL for both enantiomers in plasma. The method was applied to study the pharmacokinetics of etodolac enantiomers after the administration of a 300 and 400 mg dose of racemic drug to a healthy volunteer. Analysis of plasma samples showed higher plasma concentration of (−)-( R )-Etodolacfor both doses (300 mg dose: AUC 0–∞ 49.80 versus 4.55 ug h/mL;400 mg dose: AUC 0–∞ 63.90 versus 6.00 ug h/mL) with an (R)-(+)/(S)-(−) ratio of approximately 11. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 07317085
- Volume :
- 120
- Database :
- Academic Search Index
- Journal :
- Journal of Pharmaceutical & Biomedical Analysis
- Publication Type :
- Academic Journal
- Accession number :
- 112743220
- Full Text :
- https://doi.org/10.1016/j.jpba.2015.12.009