Synthesis and evaluations of an acid-cleavable, fluorescently labeled nucleotide as a reversible terminator for DNA sequencing.

An acid-cleavable linker based on a dimethylketal moiety was synthesized and used to connect a nucleotide with a fluorophore to produce a 3′-OH unblocked nucleotide analogue as an excellent reversible terminator for DNA sequencing by synthesis. [ABSTRACT FROM AUTHOR]