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MicroRNA-21 stimulates epithelial-to-mesenchymal transition and tumorigenesis in clear cell renal cells.
- Source :
-
Molecular Medicine Reports . 2016, Vol. 13 Issue 1, p75-82. 8p. - Publication Year :
- 2016
-
Abstract
- Clear cell renal cell carcinoma (ccRCC) metastasis may result from epithelial-to-mesenchymal transition and mesenchymal stem cells that contribute to the development of the primary tumor. In this study, it was demonstrated that microRNA-21 (miR-21) acts as an oncogenic driver of ccRCC. ccRCC spheres were isolated and it was shown that they exhibited cancer stem cell-like properties, including the formation of self-renewing spheres. Spheres showed increased expression of stem cell-related transcription factors and epithelial-mesenchymal transition (EMT) markers. In addition, secondary sphere formation capacity was assessed after miR-21 transfection. miR-21 accelerated the formation of ccRCC spheres, which shared molecular characteristics with the spontaneous ccRCC spheres. It was demonstrated that miR-21 overexpression facilitates ccRCC sphere formation. Thus, a single miRNA may have an impact on the formation of highly tumorigenic cancer spheres in kidney cancer. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 17912997
- Volume :
- 13
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Molecular Medicine Reports
- Publication Type :
- Academic Journal
- Accession number :
- 112426358
- Full Text :
- https://doi.org/10.3892/mmr.2015.4568