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Extensive macrophage accumulation in young and old Niemann–Pick C1 model mice involves the alternative, M2, activation pathway and inhibition of macrophage apoptosis.

Authors :
Deutsch, Gail
Muralidhar, Akshay
Le, Ellen
Borbon, Ivan A.
Erickson, Robert P.
Source :
Gene. Mar2016, Vol. 578 Issue 2, p242-250. 9p.
Publication Year :
2016

Abstract

We have studied the pathophysiology of lung disease which occurs in two mouse models of Niemann–Pick C1 disease. We utilized Npc1 −/− mice transgenic for normal gene expression in glia or neurons and glia at ages several fold the usual and a mouse model of the juvenile form of NPC1 , a point mutation, at one age to confirm some findings. Lung weights, as per cent of body weight, increase much more than liver and spleen weights. Although pulmonary function parameters only vary for hysteresis between young and older Npc1 −/− mice, they are markedly different than those found in normal control mice. Cholesterol accumulation continued in the older mice but sphingosine-1-phosphate was not increased. Bronchoalveolar lavage (BAL) showed a massive increase (26 ×) in the number of macrophages. Histologic examination from the older, transgenic Npc1 −/− mice showed small foci of alveolar proteinosis and evidence of hemorrhage, as well as dense macrophage accumulation. A large subset of macrophages was immunopositive for Fizz1 or arginase-1, markers of the alternative activation pathway, while no Fizz1 or arginase-1 positive macrophages were found in wild-type mice. The percentage of marker positive macrophages was relatively stable at 5–10% at various ages and within the 2 transgenic models. Phosphohistone H3 and Ki67 showed low levels of proliferation of these macrophages. Apoptosis was prominent within lung capillary endothelial cells, but limited within macrophages. Thus, activation of the alternative pathway is involved in Niemann–Pick C1 associated pulmonary macrophage accumulation, with low proliferation of these cells balanced by low levels of apoptosis. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
03781119
Volume :
578
Issue :
2
Database :
Academic Search Index
Journal :
Gene
Publication Type :
Academic Journal
Accession number :
112389602
Full Text :
https://doi.org/10.1016/j.gene.2015.12.033