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Tea polyphenols inactivate Cronobacter sakazakii isolated from powdered infant formula.

Authors :
Li, R.
Fei, P.
Man, C. X.
Lou, B. B.
Niu, J. T.
Feng, J.
Sun, L. H.
Li, M. Y.
Jiang, Y. J.
Source :
Journal of Dairy Science. Feb2016, Vol. 99 Issue 2, p1019-1028. 10p.
Publication Year :
2016

Abstract

This study evaluated the antimicrobial activity of tea polyphenols (TP) against 4 Cronobacter sakazakii strains with different sequence types (ST) isolated from powdered infant formula (PIF). The results showed that in normal saline, 5 mg/mL of TP (pH 3.44) could eliminate approximately 7.0 log cfu/mL of C. sakazakii within 1 h; in rehydrated PIF, after acidification with HCl (pH 3.55), TP showed a stronger antibacterial activity compared with the controls (malic acid, ascorbic acid, and citric acid). Further, some differences were obvious in tolerance to TP between C. sakazakii strains with different ST. The tolerance of C. sakazakii CE1 (ST4) to TP was found to be greater than that of the other 3 C. sakazakii strains (ST1, ST8, and ST64). The results of recovered test and transmission electron microscope analysis revealed that the action of TP against C. sakazakii was an irreversible bactericidal process caused by leakage of cytoplasm. Taken together, these results indicated that TP had an effective bactericidal effect against C. sakazakii, and provided a new idea for preventing and inactivating C. sakazakii in PIF. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
00220302
Volume :
99
Issue :
2
Database :
Academic Search Index
Journal :
Journal of Dairy Science
Publication Type :
Academic Journal
Accession number :
112162660
Full Text :
https://doi.org/10.3168/jds.2015-10039