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Identification and characterization of MKK7 as an upstream activator of JNK in Litopenaeus vannamei.

Authors :
Wang, Sheng
Qian, Zhe
Li, Haoyang
Lǚ, Kai
Xu, Xiaopeng
Weng, Shaoping
He, Jianguo
Li, Chaozheng
Source :
Fish & Shellfish Immunology. Jan2016, Vol. 48, p285-294. 10p.
Publication Year :
2016

Abstract

Mitogen-activated protein kinase kinase 7 (MKK7) is a key signal transduction regulator in c-Jun N-terminal kinase (JNK) signaling pathway, which is involved in a wide range of physiological and pathological processes. In this study, we described the molecular cloning of a new member of MKK7 group from Litopenaeus vannamei named as LvMKK7. The full-length cDNA of LvMKK7 was 3093 bp in length, with an open reading frame (ORF) of 1440 bp encoding a putative protein of 479 amino acids. LvMKK7 contained a conserved kinase domain of 261 amino acids in which there was a characteristic S-K-A-K-T motif as a potential target site of phosphorylation by MKKK. Moreover, subcellular localization showed LvMKK7 was located in both the cytoplasm and the nucleus of Drosophila S2 cells. Real-time PCR indicated that LvMKK7 was universally expressed in all tested tissues and its expression in hepatopancreas was responsive to the challenge of LPS, Poly (I:C), Vibrio parahaemolyticus , Staphhylococcus aureus and white spot syndrome virus (WSSV). In addition, co-immunoprecipitation assay demonstrated that LvJNK was phosphorylated and activated by LvMKK7, which suggested LvMKK7 was the upper regulator of LvJNK. Furthermore, RNAi-mediated knockdown of LvMKK7 enhanced the sensitivity of shrimps to V. parahaemolyticus infection. Overall, our results suggested that LvMKK7 may play important roles in the shrimp innate immunity. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
10504648
Volume :
48
Database :
Academic Search Index
Journal :
Fish & Shellfish Immunology
Publication Type :
Academic Journal
Accession number :
112056786
Full Text :
https://doi.org/10.1016/j.fsi.2015.12.014