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Layered molybdenum selenide stacking flower-like nanostructure coupled with guanine-rich DNA sequence for ultrasensitive ochratoxin A aptasensor application.
- Source :
-
Sensors & Actuators B: Chemical . Mar2016, Vol. 225, p391-397. 7p. - Publication Year :
- 2016
-
Abstract
- Sensitive detection of ochratoxin A (OTA) plays an important role in the field of food safety control. In this study, we are reporting for the first time an electrochemical biosensor which employs the integration of MoSe 2 nanoflowers, Au nanoparticles (AuNPs), aptamer and the guanine-rich complementary DNA sequence for the sensitive detection of OTA. MoSe 2 nanoflowers are prepared by a simple hydrothermal method and used as a promising sensing platform based on its large surface area. The methylene blue is used as a tracer and a guanine-rich complementary DNA sequence is designed to bind with the unbound OTA aptamer for signal amplification. The aptamer is immobilized on the AuNPs/MoSe 2 modified electrode surface through Au–S interaction. Since OTA can competitively bind with the aptamer due to their high affinity, the presence of OTA will induce the decrease of guanine-rich DNA on the electrode surface, in turn reduces the signal of methylene blue which can specifically adsorb to the guanine units of DNA. The proposed assay shows a good linear relationship within the range of 0.0001–1 nM with a detection limit of 0.08 pM for OTA. The developed biosensor has been applied to determine OTA concentration in wine samples. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09254005
- Volume :
- 225
- Database :
- Academic Search Index
- Journal :
- Sensors & Actuators B: Chemical
- Publication Type :
- Academic Journal
- Accession number :
- 111877649
- Full Text :
- https://doi.org/10.1016/j.snb.2015.11.070