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The Arabidopsis thaliana K+-uptake permease 7 (AtKUP7) contains a functional cytosolic adenylate cyclase catalytic centre.
- Source :
-
FEBS Letters . Dec2015 Part B, Vol. 589 Issue 24PartB, p3848-3852. 5p. - Publication Year :
- 2015
-
Abstract
- Adenylate cyclases (ACs) catalyse the formation of the second messenger cyclic adenosine 3′,5′-monophosphate (cAMP) from adenosine 5′-triphosphate (ATP). Although cAMP is increasingly recognised as an important signalling molecule in higher plants, ACs have remained somewhat elusive. Here we used a search motif derived from experimentally tested guanylyl cyclases (GCs), substituted the residues essential for substrate specificity and identified the Arabidopsis thaliana K + -uptake permease 7 (AtKUP7) as one of several candidate ACs. Firstly, we show that a recombinant N-terminal, cytosolic domain of AtKUP7 1-100 is able to complement the AC-deficient mutant cyaA in Escherichia coli and thus restoring the fermentation of lactose, and secondly, we demonstrate with both enzyme immunoassays and mass spectrometry that a recombinant AtKUP7 1-100 generates cAMP in vitro. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00145793
- Volume :
- 589
- Issue :
- 24PartB
- Database :
- Academic Search Index
- Journal :
- FEBS Letters
- Publication Type :
- Academic Journal
- Accession number :
- 111564001
- Full Text :
- https://doi.org/10.1016/j.febslet.2015.11.038