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Effects of olanzapine on LPS-induced inflammation in rat primary glia cells.

Authors :
Faour-Nmarne, Caroline
Azab, Abed N.
Source :
Innate Immunity. Jan2016, Vol. 22 Issue 1, p40-50. 11p.
Publication Year :
2016

Abstract

Olanzapine (OLZ) is an atypical antipsychotic drug that also has mood-stabilizing effects. The mechanism of action of OLZ is not fully understood. Accumulating data suggest that inflammation plays a role in the pathophysiology of mental disorders and that psychotropic drugs exhibit some anti-inflammatory properties. This study was undertaken to examine the effects of OLZ on LPS-induced inflammation in rat primary glia cells. Glia cells were extracted from newborn rat brains. OLZ (1 or 50 µM) was added to culture medium at 6 or 72 h before addition of LPS for another 18 h, and levels of IL-10, prostaglandin (PG) E2, NO and TNF-α, and expression of cyclo-oxygensase (COX)-2 and inducible NO synthase (iNOS) were determined. Treatment with 50 µM OLZ (but not 1 µM) significantly decreased LPS-induced secretion of IL-10, PGE2 and TNF-α. In contrast, 50 µM OLZ significantly increased NO levels. OLZ did not alter the expression of COX-2 or iNOS in LPS-treated cells. These results suggest that OLZ differently affects the secretion of inflammatory mediators. Most of the significant effects of OLZ were obtained when 50 µM was used, which is a high and probably therapeutically irrelevant concentration. Therefore, under the conditions used in the present study OLZ seemed to lack a potent anti-inflammatory effect. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
17534259
Volume :
22
Issue :
1
Database :
Academic Search Index
Journal :
Innate Immunity
Publication Type :
Academic Journal
Accession number :
111546414
Full Text :
https://doi.org/10.1177/1753425915613425