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High affinity IgM+ memory B cells are generated through a germinal center-dependent pathway.
- Source :
-
Molecular Immunology . Dec2015, Vol. 68 Issue 2 Part C, p617-627. 11p. - Publication Year :
- 2015
-
Abstract
- During a T cell-dependent immune response, B cells undergo clonal expansion and selection and the induction of isotype switching and somatic hypermutation (SHM). Although somatically mutated IgM + memory B cells have been reported, it has not been established whether they are really high affinity B cells. We tracked (4-hydroxy-3-nitrophenyl) acetyl hapten-specific GC B cells from normal immunized mice based on affinity of their B cell receptor (BCR) and performed BCR sequence analysis. SHM was evident by day 7 postimmunization and increased with time, such that high affinity IgM + as well as IgG + memory B cells continued to be generated up to day 42. In contrast, class-switch recombination (CSR) was almost completed by day 7 and then the ratio of IgG1 + /IgM + GC B cells remained unchanged. Together these findings suggest that IgM + B cells undergo SHM in the GC to generate high affinity IgM + memory cells and that this process continues even after CSR is accomplished. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 01615890
- Volume :
- 68
- Issue :
- 2 Part C
- Database :
- Academic Search Index
- Journal :
- Molecular Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 111421226
- Full Text :
- https://doi.org/10.1016/j.molimm.2015.10.003